Hormonal Control of Polyribosome Formation in Barley Aleurone Layers

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Hormonal control of messenger ribonucleic acid metabolism in barley aleurone layers.

Ribonucleic acid containing poly(adenylic acid) [poly(A)-RNA] is present in barley aleurone layers. This poly(A)-RNA becomes labeled with radioactive precursors of RNA during the incubation of isolated aleurone layers with or without gibberellic acid. However, the rate of synthesis of poly(A)-RNA is enhanced by gibberellic acid. This enhancement begins within 3-4 hr of addition of the hormone a...

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Hormonal control of orthophosphate incorporation into phospholipids of barley aleurone layers.

Gibberellic acid added to isolated barley aleurone layers enhances orthophosphate incorporation into chloroform-methanol-soluble compounds. The effect is measurable at 4 to 6 hours after the addition of gibberellic acid and reaches a maximum after 8 to 12 hours. The increase in the rate of orthophosphate incorporation is 3- to 5-fold over the rate in control layers incubated without gibberellic...

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Control of nitrate reductase activity in barley aleurone layers.

Nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers has been determined in the intact tissue, using two different methods. The first method measures the rate of appearance of H(2) (18)O produced during the reduction of KN(18)O(3). The second assay measures excreted nitrite resulting from nitrate reduction under anaerobic conditions. Nitrite production in this ...

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Osmotic Regulation of Α-amylase Synthesis and Polyribosome Formation in Aleurone Cells of Barley

Water stress inhibits the gibberellic acid (GA(3))-induced synthesis of alpha-amylase in aleurone layers of barley (Hordeum vulgare L.). Electron microscope evidence indicates that the effect of water stress induced by 0.6 M solutions of polyethylene glycol (PEG) is to reduce the binding of ribosomes to the endoplasmic reticulum. This was confirmed by sucrose density gradient centrifugation of ...

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Hormonal regulation of the development of protease and carboxypeptidase activities in barley aleurone layers.

Carboxypeptidase and protease activities of hormone-treated barley (Hordeum vulgare cv Himalaya) aleurone layers were investigated using the substrates N-carbobenzoxy-Ala-Phe and hemoglobin. A differential effect of gibberellic acid (GA(3)) on these activities was observed. The carboxypeptidase activity develops in the aleurone layers during imbibition without the addition of hormone, while the...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1972

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.49.3.348